Freeze-substitution transmission electron microscopy of gentian shoot tips cryopreserved at ultra low temperatures
نویسندگان
چکیده
منابع مشابه
Tandem high-pressure freezing and quick freeze substitution of plant tissues for transmission electron microscopy.
Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-friendly technique. Traditional sample preparation for TEM used chemical fixatives to preserve cellu...
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OBJECTIVE Recent publications questioned the validity of endothelial cell (EC) culture studies of glycocalyx (GCX) function because of findings that GCX in vitro may be substantially thinner than GCX in vivo. The assessment of thickness differences is complicated by GCX collapse during dehydration for traditional electron microscopy. We measured in vitro GCX thickness using rapid freezing/freez...
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Cryopreservation is a way to store elite quality plant germplasms. The exact mechanism of stress tolerance during cryopreservation is unknown. Unavavailability of a detailed protocol for understanding the molecular genetics of plant cryostress is a major obstacle in plant cryobiology research. This paper describes the methods of extraction of total RNA from cryogenically stored plant tissues ac...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2018
ISSN: 1342-4580,1347-6114
DOI: 10.5511/plantbiotechnology.18.0718a